Construct: shRNA TRCN0000082170

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_173780.2-617s1c1
DNA Barcode:
CCAAATAAGATGAGTGGACTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Klf8 (245671)
Gene Description:
Kruppel-like factor 8
Transcript:
RefSeq NM_173780.2 (NON-CURRENT)
Match location:
Position 617 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000082170)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAAATAAGATGAGTGGACTA
Hairpin Sequence:
5'-CCGG-CCAAATAAGATGAGTGGACTA-CTCGAG-TAGTCCACTCATCTTATTTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAAATAAGATGAGTGGACTACTCGAGTAGTCCACTCATCTTATTTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAAATAAGATGAGTGGACTACTCGAGTAGTCCACTCATCTTATTTGG-3'

Other clones with same target sequence:

(none)