Construct: shRNA TRCN0000082203

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_021455.2-3407s1c1
DNA Barcode:
CCTCTGGAATACTGGAATTAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Mlxipl (58805)
Gene Description:
MLX interacting protein-like
Transcript:
RefSeq NM_021455.2 (NON-CURRENT)
Match location:
Position 3407 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000082203)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTCTGGAATACTGGAATTAA
Hairpin Sequence:
5'-CCGG-CCTCTGGAATACTGGAATTAA-CTCGAG-TTAATTCCAGTATTCCAGAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTCTGGAATACTGGAATTAACTCGAGTTAATTCCAGTATTCCAGAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTCTGGAATACTGGAATTAACTCGAGTTAATTCCAGTATTCCAGAGG-3'

Other clones with same target sequence:

(none)