Construct: shRNA TRCN0000082220

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_027949.1-1456s1c1
DNA Barcode:
CAGCAAGAAATGGGAATGTAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Phf7 (71838)
Gene Description:
PHD finger protein 7
Transcript:
RefSeq NM_027949.1 (CURRENT)
Match location:
Position 1456 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000082220)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAGCAAGAAATGGGAATGTAA
Hairpin Sequence:
5'-CCGG-CAGCAAGAAATGGGAATGTAA-CTCGAG-TTACATTCCCATTTCTTGCTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAGCAAGAAATGGGAATGTAACTCGAGTTACATTCCCATTTCTTGCTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAGCAAGAAATGGGAATGTAACTCGAGTTACATTCCCATTTCTTGCTG-3'

Other clones with same target sequence:

  1. TRCN0000301888