Construct: shRNA TRCN0000082235

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_031184.1-299s1c1
DNA Barcode:
CCTAAAGCTGAGCATCACCAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Glis2 (83396)
Gene Description:
GLIS family zinc finger 2
Transcript:
RefSeq NM_031184.1 (NON-CURRENT)
Match location:
Position 81 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000082235)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTAAAGCTGAGCATCACCAA
Hairpin Sequence:
5'-CCGG-CCTAAAGCTGAGCATCACCAA-CTCGAG-TTGGTGATGCTCAGCTTTAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTAAAGCTGAGCATCACCAACTCGAGTTGGTGATGCTCAGCTTTAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTAAAGCTGAGCATCACCAACTCGAGTTGGTGATGCTCAGCTTTAGG-3'

Other clones with same target sequence:

(none)