Construct: shRNA TRCN0000082281

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_031391.1-106s1c1
DNA Barcode:
GATGACGGAGTGGATGAACAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Gtf2a1 (83602)
Gene Description:
general transcription factor II A, 1
Transcript:
RefSeq NM_031391.1 (NON-CURRENT)
Match location:
Position 106 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000082281)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GATGACGGAGTGGATGAACAA
Hairpin Sequence:
5'-CCGG-GATGACGGAGTGGATGAACAA-CTCGAG-TTGTTCATCCACTCCGTCATC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGATGACGGAGTGGATGAACAACTCGAGTTGTTCATCCACTCCGTCATCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGATGACGGAGTGGATGAACAACTCGAGTTGTTCATCCACTCCGTCATC-3'

Other clones with same target sequence:

(none)