Construct: shRNA TRCN0000082349

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_371497.3-1169s1c1
DNA Barcode:
CCCAGTCCTTATCCAAGTATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
DKFZp686K16132 (388957)
Gene Description:
hypothetical LOC388957
Transcript:
RefSeq XM_371497.3 (NON-CURRENT)
Match location:
Position 1169 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000082349)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCAGTCCTTATCCAAGTATT
Hairpin Sequence:
5'-CCGG-CCCAGTCCTTATCCAAGTATT-CTCGAG-AATACTTGGATAAGGACTGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCAGTCCTTATCCAAGTATTCTCGAGAATACTTGGATAAGGACTGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCAGTCCTTATCCAAGTATTCTCGAGAATACTTGGATAAGGACTGGG-3'

Other clones with same target sequence:

(none)