Construct: shRNA TRCN0000082354

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_370975.2-641s1c1
DNA Barcode:
CGGAGGCGATGGGAGAGAATA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
LOC388259 (388259)
Gene Description:
hypothetical LOC388259
Transcript:
RefSeq XM_370975.2 (NON-CURRENT)
Match location:
Position 641 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000082354)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGAGGCGATGGGAGAGAATA
Hairpin Sequence:
5'-CCGG-CGGAGGCGATGGGAGAGAATA-CTCGAG-TATTCTCTCCCATCGCCTCCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGAGGCGATGGGAGAGAATACTCGAGTATTCTCTCCCATCGCCTCCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGAGGCGATGGGAGAGAATACTCGAGTATTCTCTCCCATCGCCTCCG-3'

Other clones with same target sequence:

(none)