Construct: shRNA TRCN0000082362

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_495804.1-760s1c1
DNA Barcode:
AGGGAGTTCAACTCTGAGAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
LOC439955 (439955)
Gene Description:
similar to general transcription factor II, i isoform 1; BTK-associated protein, 135kD; Williams-Beuren syndrome chromosome region 6; Bruton tyrosine kinase-associated protein 135
Transcript:
RefSeq XM_495804.1 (NON-CURRENT)
Match location:
Position 760 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000082362)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGGGAGTTCAACTCTGAGAAA
Hairpin Sequence:
5'-CCGG-AGGGAGTTCAACTCTGAGAAA-CTCGAG-TTTCTCAGAGTTGAACTCCCT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGGGAGTTCAACTCTGAGAAACTCGAGTTTCTCAGAGTTGAACTCCCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGGGAGTTCAACTCTGAGAAACTCGAGTTTCTCAGAGTTGAACTCCCT-3'

Other clones with same target sequence:

(none)