Construct: shRNA TRCN0000082363
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- XM_372273.3-1293s1c1
- DNA Barcode:
- GCCGACTGTAACATGTTTCAT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- LOC389906 (389906)
- Gene Description:
- zinc finger protein 839 pseudogene
- Transcript:
- RefSeq XM_372273.3 (NON-CURRENT)
- Match location:
- Position 1293 (3UTR)
Vector Information:
- Vector Backbone:
- pLKO.1
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000082363)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- GCCGACTGTAACATGTTTCAT
- Hairpin Sequence:
-
5'-CCGG-GCCGACTGTAACATGTTTCAT-CTCGAG-ATGAAACATGTTACAGTCGGC-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGGCCGACTGTAACATGTTTCATCTCGAGATGAAACATGTTACAGTCGGCTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAAGCCGACTGTAACATGTTTCATCTCGAGATGAAACATGTTACAGTCGGC-3'
Other clones with same target sequence:
(none)
