Construct: shRNA TRCN0000082383

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_496720.1-200s1c1
DNA Barcode:
CCCTGGACAAAGATCGTCTCA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
LOC441047 (441047)
Gene Description:
similar to Adenylate kinase isoenzyme 4, mitochondrial (ATP-AMP transphosphorylase)
Transcript:
RefSeq XM_496720.1 (NON-CURRENT)
Match location:
Position 200 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000082383)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCTGGACAAAGATCGTCTCA
Hairpin Sequence:
5'-CCGG-CCCTGGACAAAGATCGTCTCA-CTCGAG-TGAGACGATCTTTGTCCAGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCTGGACAAAGATCGTCTCACTCGAGTGAGACGATCTTTGTCCAGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCTGGACAAAGATCGTCTCACTCGAGTGAGACGATCTTTGTCCAGGG-3'

Other clones with same target sequence:

(none)