Construct: shRNA TRCN0000082387

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_496720.1-102s1c1
DNA Barcode:
CCATGTGATCACATACCTAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
LOC441047 (441047)
Gene Description:
similar to Adenylate kinase isoenzyme 4, mitochondrial (ATP-AMP transphosphorylase)
Transcript:
RefSeq XM_496720.1 (NON-CURRENT)
Match location:
Position 102 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000082387)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCATGTGATCACATACCTAAT
Hairpin Sequence:
5'-CCGG-CCATGTGATCACATACCTAAT-CTCGAG-ATTAGGTATGTGATCACATGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCATGTGATCACATACCTAATCTCGAGATTAGGTATGTGATCACATGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCATGTGATCACATACCTAATCTCGAGATTAGGTATGTGATCACATGG-3'

Other clones with same target sequence:

(none)