Construct: shRNA TRCN0000082421

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_371588.2-616s1c1
DNA Barcode:
ACAGCCAGAATCATAGACAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
LOC389069 (389069)
Gene Description:
similar to selenophosphate synthetase
Transcript:
RefSeq XM_371588.2 (NON-CURRENT)
Match location:
Position 616 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000082421)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACAGCCAGAATCATAGACAAA
Hairpin Sequence:
5'-CCGG-ACAGCCAGAATCATAGACAAA-CTCGAG-TTTGTCTATGATTCTGGCTGT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACAGCCAGAATCATAGACAAACTCGAGTTTGTCTATGATTCTGGCTGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACAGCCAGAATCATAGACAAACTCGAGTTTGTCTATGATTCTGGCTGT-3'

Other clones with same target sequence:

(none)