Construct: shRNA TRCN0000082423

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_018221.1-2102s1c1
DNA Barcode:
CCTCTATTTCTATTGGGATAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MOB1A (55233)
Gene Description:
MOB kinase activator 1A
Transcript:
RefSeq NM_018221.1 (NON-CURRENT)
Match location:
Position 2102 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000082423)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTCTATTTCTATTGGGATAT
Hairpin Sequence:
5'-CCGG-CCTCTATTTCTATTGGGATAT-CTCGAG-ATATCCCAATAGAAATAGAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTCTATTTCTATTGGGATATCTCGAGATATCCCAATAGAAATAGAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTCTATTTCTATTGGGATATCTCGAGATATCCCAATAGAAATAGAGG-3'

Other clones with same target sequence:

(none)