Construct: shRNA TRCN0000082451

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_376921.1-266s1c1
DNA Barcode:
GCATGTGATAAAGCAGGTGGA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
STKLD1 (169436)
Gene Description:
serine/threonine kinase like domain containing 1
Transcript:
RefSeq XM_376921.1 (NON-CURRENT)
Match location:
Position 266 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000082451)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCATGTGATAAAGCAGGTGGA
Hairpin Sequence:
5'-CCGG-GCATGTGATAAAGCAGGTGGA-CTCGAG-TCCACCTGCTTTATCACATGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCATGTGATAAAGCAGGTGGACTCGAGTCCACCTGCTTTATCACATGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCATGTGATAAAGCAGGTGGACTCGAGTCCACCTGCTTTATCACATGC-3'

Other clones with same target sequence:

(none)