Construct: shRNA TRCN0000082509
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- XM_373815.2-237s1c1
- DNA Barcode:
- CGTGGGATGGTTCCTTTCAAA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- LOC388573 (388573)
- Gene Description:
- similar to General transcription factor II-I (GTFII-I) (TFII-I) (Bruton tyrosine kinase-associated protein-135) (BTK-associated protein-135) (BAP-135)
- Transcript:
- RefSeq XM_373815.2 (NON-CURRENT)
- Match location:
- Position 237 (CDS)
Vector Information:
- Vector Backbone:
- pLKO.1
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000082509)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- CGTGGGATGGTTCCTTTCAAA
- Hairpin Sequence:
-
5'-CCGG-CGTGGGATGGTTCCTTTCAAA-CTCGAG-TTTGAAAGGAACCATCCCACG-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGCGTGGGATGGTTCCTTTCAAACTCGAGTTTGAAAGGAACCATCCCACGTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAACGTGGGATGGTTCCTTTCAAACTCGAGTTTGAAAGGAACCATCCCACG-3'
Other clones with same target sequence:
(none)
