Construct: shRNA TRCN0000082512

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_373815.2-424s1c1
DNA Barcode:
GCCTCAACCTTCCAGGCTGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
LOC388573 (388573)
Gene Description:
similar to General transcription factor II-I (GTFII-I) (TFII-I) (Bruton tyrosine kinase-associated protein-135) (BTK-associated protein-135) (BAP-135)
Transcript:
RefSeq XM_373815.2 (NON-CURRENT)
Match location:
Position 424 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000082512)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCTCAACCTTCCAGGCTGAA
Hairpin Sequence:
5'-CCGG-GCCTCAACCTTCCAGGCTGAA-CTCGAG-TTCAGCCTGGAAGGTTGAGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCTCAACCTTCCAGGCTGAACTCGAGTTCAGCCTGGAAGGTTGAGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCTCAACCTTCCAGGCTGAACTCGAGTTCAGCCTGGAAGGTTGAGGC-3'

Other clones with same target sequence:

(none)