Construct: shRNA TRCN0000082567

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_497921.1-933s1c1
DNA Barcode:
CGTAAATGCTTTCAAGTTCAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
FLT1P1 (391533)
Gene Description:
fms related tyrosine kinase 1 pseudogene 1
Transcript:
RefSeq XM_497921.1 (NON-CURRENT)
Match location:
Position 933 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000082567)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGTAAATGCTTTCAAGTTCAT
Hairpin Sequence:
5'-CCGG-CGTAAATGCTTTCAAGTTCAT-CTCGAG-ATGAACTTGAAAGCATTTACG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGTAAATGCTTTCAAGTTCATCTCGAGATGAACTTGAAAGCATTTACGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGTAAATGCTTTCAAGTTCATCTCGAGATGAACTTGAAAGCATTTACG-3'

Other clones with same target sequence:

(none)