Construct: shRNA TRCN0000082578

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_201548.3-1853s1c1
DNA Barcode:
CGCACTTCTATGAGAATCTAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CERKL (375298)
Gene Description:
ceramide kinase like
Transcript:
RefSeq NM_201548.3 (NON-CURRENT)
Match location:
Position 1853 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000082578)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGCACTTCTATGAGAATCTAA
Hairpin Sequence:
5'-CCGG-CGCACTTCTATGAGAATCTAA-CTCGAG-TTAGATTCTCATAGAAGTGCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGCACTTCTATGAGAATCTAACTCGAGTTAGATTCTCATAGAAGTGCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGCACTTCTATGAGAATCTAACTCGAGTTAGATTCTCATAGAAGTGCG-3'

Other clones with same target sequence:

(none)