Construct: shRNA TRCN0000082580

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_201548.3-1564s1c1
DNA Barcode:
CCAAGACTTATCAGTCTTTAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CERKL (375298)
Gene Description:
ceramide kinase like
Transcript:
RefSeq NM_201548.3 (NON-CURRENT)
Match location:
Position 1564 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000082580)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAAGACTTATCAGTCTTTAT
Hairpin Sequence:
5'-CCGG-CCAAGACTTATCAGTCTTTAT-CTCGAG-ATAAAGACTGATAAGTCTTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAAGACTTATCAGTCTTTATCTCGAGATAAAGACTGATAAGTCTTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAAGACTTATCAGTCTTTATCTCGAGATAAAGACTGATAAGTCTTGG-3'

Other clones with same target sequence:

(none)