Construct: shRNA TRCN0000082592

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_372274.2-705s1c1
DNA Barcode:
CCTTCAAGACTTCGACACGCT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
LOC389907 (389907)
Gene Description:
similar to Serine/threonine-protein kinase PRKX (Protein kinase PKX1)
Transcript:
RefSeq XM_372274.2 (NON-CURRENT)
Match location:
Position 705 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000082592)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTTCAAGACTTCGACACGCT
Hairpin Sequence:
5'-CCGG-CCTTCAAGACTTCGACACGCT-CTCGAG-AGCGTGTCGAAGTCTTGAAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTTCAAGACTTCGACACGCTCTCGAGAGCGTGTCGAAGTCTTGAAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTTCAAGACTTCGACACGCTCTCGAGAGCGTGTCGAAGTCTTGAAGG-3'

Other clones with same target sequence:

(none)