Construct: shRNA TRCN0000082637

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_498262.1-30s1c1
DNA Barcode:
CATCCTAGAGTCCCTGGAGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
LOC442370 (442370)
Gene Description:
similar to cAMP-dependent protein kinase type I-beta regulatory chain
Transcript:
RefSeq XM_498262.1 (NON-CURRENT)
Match location:
Position 30 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000082637)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CATCCTAGAGTCCCTGGAGAA
Hairpin Sequence:
5'-CCGG-CATCCTAGAGTCCCTGGAGAA-CTCGAG-TTCTCCAGGGACTCTAGGATG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCATCCTAGAGTCCCTGGAGAACTCGAGTTCTCCAGGGACTCTAGGATGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACATCCTAGAGTCCCTGGAGAACTCGAGTTCTCCAGGGACTCTAGGATG-3'

Other clones with same target sequence:

(none)