Construct: shRNA TRCN0000082638

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_022140.2-2055s1c1
DNA Barcode:
CCACGAAGTTACCGCCAGTAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
EPB41L4A (64097)
Gene Description:
erythrocyte membrane protein band 4.1 like 4A
Transcript:
RefSeq NM_022140.2 (NON-CURRENT)
Match location:
Position 2055 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000082638)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCACGAAGTTACCGCCAGTAT
Hairpin Sequence:
5'-CCGG-CCACGAAGTTACCGCCAGTAT-CTCGAG-ATACTGGCGGTAACTTCGTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCACGAAGTTACCGCCAGTATCTCGAGATACTGGCGGTAACTTCGTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCACGAAGTTACCGCCAGTATCTCGAGATACTGGCGGTAACTTCGTGG-3'

Other clones with same target sequence:

(none)