Construct: shRNA TRCN0000082668

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_144688.2-3725s1c1
DNA Barcode:
CCAGTAATGGATTAAGCACTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CCDC155 (147872)
Gene Description:
coiled-coil domain containing 155
Transcript:
RefSeq NM_144688.2 (NON-CURRENT)
Match location:
Position 3725 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000082668)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGTAATGGATTAAGCACTA
Hairpin Sequence:
5'-CCGG-CCAGTAATGGATTAAGCACTA-CTCGAG-TAGTGCTTAATCCATTACTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGTAATGGATTAAGCACTACTCGAGTAGTGCTTAATCCATTACTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGTAATGGATTAAGCACTACTCGAGTAGTGCTTAATCCATTACTGG-3'

Other clones with same target sequence:

(none)