Construct: shRNA TRCN0000082692

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_015361.2-1175s1c1
DNA Barcode:
CAGATGAGAATACGTTTGAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
R3HDM1 (23518)
Gene Description:
R3H domain containing 1
Transcript:
RefSeq NM_015361.2 (NON-CURRENT)
Match location:
Position 1175 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000082692)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAGATGAGAATACGTTTGAAA
Hairpin Sequence:
5'-CCGG-CAGATGAGAATACGTTTGAAA-CTCGAG-TTTCAAACGTATTCTCATCTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAGATGAGAATACGTTTGAAACTCGAGTTTCAAACGTATTCTCATCTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAGATGAGAATACGTTTGAAACTCGAGTTTCAAACGTATTCTCATCTG-3'

Other clones with same target sequence:

  1. TRCN0000291108