Construct: shRNA TRCN0000082694

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_017762.1-1028s1c1
DNA Barcode:
CCAGAGGATTTGTAATGCAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MTMR10 (54893)
Gene Description:
myotubularin related protein 10
Transcript:
RefSeq NM_017762.1 (NON-CURRENT)
Match location:
Position 1028 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000082694)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGAGGATTTGTAATGCAAT
Hairpin Sequence:
5'-CCGG-CCAGAGGATTTGTAATGCAAT-CTCGAG-ATTGCATTACAAATCCTCTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGAGGATTTGTAATGCAATCTCGAGATTGCATTACAAATCCTCTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGAGGATTTGTAATGCAATCTCGAGATTGCATTACAAATCCTCTGG-3'

Other clones with same target sequence:

(none)