Construct: shRNA TRCN0000082733

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_088142.5-903s1c1
DNA Barcode:
CAGAGAAACATTCACACACAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ATP6V0E2 (155066)
Gene Description:
ATPase H+ transporting V0 subunit e2
Transcript:
RefSeq XM_088142.5 (NON-CURRENT)
Match location:
Position 903 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000082733)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAGAGAAACATTCACACACAA
Hairpin Sequence:
5'-CCGG-CAGAGAAACATTCACACACAA-CTCGAG-TTGTGTGTGAATGTTTCTCTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAGAGAAACATTCACACACAACTCGAGTTGTGTGTGAATGTTTCTCTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAGAGAAACATTCACACACAACTCGAGTTGTGTGTGAATGTTTCTCTG-3'

Other clones with same target sequence:

  1. TRCN0000307213