Construct: shRNA TRCN0000082753

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_178532.2-1451s1c1
DNA Barcode:
GCACTGGTATTCTCAGTGTTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RNF180 (285671)
Gene Description:
ring finger protein 180
Transcript:
RefSeq NM_178532.2 (NON-CURRENT)
Match location:
Position 1451 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000082753)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCACTGGTATTCTCAGTGTTT
Hairpin Sequence:
5'-CCGG-GCACTGGTATTCTCAGTGTTT-CTCGAG-AAACACTGAGAATACCAGTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCACTGGTATTCTCAGTGTTTCTCGAGAAACACTGAGAATACCAGTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCACTGGTATTCTCAGTGTTTCTCGAGAAACACTGAGAATACCAGTGC-3'

Other clones with same target sequence:

(none)