Construct: shRNA TRCN0000082787

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_152428.3-1215s1c1
DNA Barcode:
CCTCAGAAGACCTCCATGAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
FRMPD2 (143162)
Gene Description:
FERM and PDZ domain containing 2
Transcript:
RefSeq NM_152428.3 (NON-CURRENT)
Match location:
Position 1215 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000082787)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTCAGAAGACCTCCATGAAT
Hairpin Sequence:
5'-CCGG-CCTCAGAAGACCTCCATGAAT-CTCGAG-ATTCATGGAGGTCTTCTGAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTCAGAAGACCTCCATGAATCTCGAGATTCATGGAGGTCTTCTGAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTCAGAAGACCTCCATGAATCTCGAGATTCATGGAGGTCTTCTGAGG-3'

Other clones with same target sequence:

(none)