Construct: shRNA TRCN0000082793

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_194449.1-3802s1c1
DNA Barcode:
CCGAGCTGTTTAACAAATAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PHLPP1 (23239)
Gene Description:
PH domain and leucine rich repeat protein phosphatase 1
Transcript:
RefSeq NM_194449.1 (NON-CURRENT)
Match location:
Position 3802 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000082793)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCGAGCTGTTTAACAAATAAA
Hairpin Sequence:
5'-CCGG-CCGAGCTGTTTAACAAATAAA-CTCGAG-TTTATTTGTTAAACAGCTCGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCGAGCTGTTTAACAAATAAACTCGAGTTTATTTGTTAAACAGCTCGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCGAGCTGTTTAACAAATAAACTCGAGTTTATTTGTTAAACAGCTCGG-3'

Other clones with same target sequence:

  1. TRCN0000363575