Construct: shRNA TRCN0000082795

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_194449.1-2434s1c1
DNA Barcode:
CGCTGTCCTTTGTCATATCAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PHLPP1 (23239)
Gene Description:
PH domain and leucine rich repeat protein phosphatase 1
Transcript:
RefSeq NM_194449.1 (NON-CURRENT)
Match location:
Position 2434 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000082795)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGCTGTCCTTTGTCATATCAA
Hairpin Sequence:
5'-CCGG-CGCTGTCCTTTGTCATATCAA-CTCGAG-TTGATATGACAAAGGACAGCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGCTGTCCTTTGTCATATCAACTCGAGTTGATATGACAAAGGACAGCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGCTGTCCTTTGTCATATCAACTCGAGTTGATATGACAAAGGACAGCG-3'

Other clones with same target sequence:

(none)