Construct: shRNA TRCN0000082798

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_000493.2-3123s1c1
DNA Barcode:
CCTCACTTATTAAAGCACAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
COL10A1 (1300)
Gene Description:
collagen type X alpha 1 chain
Transcript:
RefSeq NM_000493.2 (NON-CURRENT)
Match location:
Position 3123 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000082798)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTCACTTATTAAAGCACAAA
Hairpin Sequence:
5'-CCGG-CCTCACTTATTAAAGCACAAA-CTCGAG-TTTGTGCTTTAATAAGTGAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTCACTTATTAAAGCACAAACTCGAGTTTGTGCTTTAATAAGTGAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTCACTTATTAAAGCACAAACTCGAGTTTGTGCTTTAATAAGTGAGG-3'

Other clones with same target sequence:

(none)