Construct: shRNA TRCN0000082813

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_201378.1-14206s1c1
DNA Barcode:
GCCTTCCATGTCGGCCTCTAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PLEC (5339)
Gene Description:
plectin
Transcript:
RefSeq NM_201378.1 (NON-CURRENT)
Match location:
Position 14206 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000082813)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCTTCCATGTCGGCCTCTAA
Hairpin Sequence:
5'-CCGG-GCCTTCCATGTCGGCCTCTAA-CTCGAG-TTAGAGGCCGACATGGAAGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCTTCCATGTCGGCCTCTAACTCGAGTTAGAGGCCGACATGGAAGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCTTCCATGTCGGCCTCTAACTCGAGTTAGAGGCCGACATGGAAGGC-3'

Other clones with same target sequence:

  1. TRCN0000315848