Construct: shRNA TRCN0000082814

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_201378.1-1641s1c1
DNA Barcode:
GCACCAGTCCATCGAAGAATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PLEC (5339)
Gene Description:
plectin
Transcript:
RefSeq NM_201378.1 (NON-CURRENT)
Match location:
Position 1641 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000082814)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCACCAGTCCATCGAAGAATT
Hairpin Sequence:
5'-CCGG-GCACCAGTCCATCGAAGAATT-CTCGAG-AATTCTTCGATGGACTGGTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCACCAGTCCATCGAAGAATTCTCGAGAATTCTTCGATGGACTGGTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCACCAGTCCATCGAAGAATTCTCGAGAATTCTTCGATGGACTGGTGC-3'

Other clones with same target sequence:

  1. TRCN0000315845