Construct: shRNA TRCN0000082868

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_000195.2-2860s1c1
DNA Barcode:
GCTCAATAAATGTTGGTCATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
HPS1 (3257)
Gene Description:
HPS1 biogenesis of lysosomal organelles complex 3 subunit 1
Transcript:
RefSeq NM_000195.2 (NON-CURRENT)
Match location:
Position 2860 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000082868)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTCAATAAATGTTGGTCATT
Hairpin Sequence:
5'-CCGG-GCTCAATAAATGTTGGTCATT-CTCGAG-AATGACCAACATTTATTGAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTCAATAAATGTTGGTCATTCTCGAGAATGACCAACATTTATTGAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTCAATAAATGTTGGTCATTCTCGAGAATGACCAACATTTATTGAGC-3'

Other clones with same target sequence:

(none)