Construct: shRNA TRCN0000082873

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_000422.1-1228s1c1
DNA Barcode:
CACCTGACTCAGTACAAGAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
KRT17 (3872)
Gene Description:
keratin 17
Transcript:
RefSeq NM_000422.1 (NON-CURRENT)
Match location:
Position 1228 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000082873)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CACCTGACTCAGTACAAGAAA
Hairpin Sequence:
5'-CCGG-CACCTGACTCAGTACAAGAAA-CTCGAG-TTTCTTGTACTGAGTCAGGTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCACCTGACTCAGTACAAGAAACTCGAGTTTCTTGTACTGAGTCAGGTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACACCTGACTCAGTACAAGAAACTCGAGTTTCTTGTACTGAGTCAGGTG-3'

Other clones with same target sequence:

(none)