Construct: shRNA TRCN0000082894

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_198270.2-4506s1c1
DNA Barcode:
CCGAGTGATGACTCCATCATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NHS (4810)
Gene Description:
NHS actin remodeling regulator
Transcript:
RefSeq NM_198270.2 (NON-CURRENT)
Match location:
Position 4506 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000082894)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCGAGTGATGACTCCATCATT
Hairpin Sequence:
5'-CCGG-CCGAGTGATGACTCCATCATT-CTCGAG-AATGATGGAGTCATCACTCGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCGAGTGATGACTCCATCATTCTCGAGAATGATGGAGTCATCACTCGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCGAGTGATGACTCCATCATTCTCGAGAATGATGGAGTCATCACTCGG-3'

Other clones with same target sequence:

(none)