Construct: shRNA TRCN0000082903

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_000256.2-3938s1c1
DNA Barcode:
CCTCCCTACTGTTGGATGTAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MYBPC3 (4607)
Gene Description:
myosin binding protein C3
Transcript:
RefSeq NM_000256.2 (NON-CURRENT)
Match location:
Position 3938 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000082903)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTCCCTACTGTTGGATGTAT
Hairpin Sequence:
5'-CCGG-CCTCCCTACTGTTGGATGTAT-CTCGAG-ATACATCCAACAGTAGGGAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTCCCTACTGTTGGATGTATCTCGAGATACATCCAACAGTAGGGAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTCCCTACTGTTGGATGTATCTCGAGATACATCCAACAGTAGGGAGG-3'

Other clones with same target sequence:

(none)