Construct: shRNA TRCN0000082965

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_000492.2-4048s1c1
DNA Barcode:
CCCTATGAACAGTGGAGTGAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CFTR (1080)
Gene Description:
CF transmembrane conductance regulator
Transcript:
RefSeq NM_000492.2 (NON-CURRENT)
Match location:
Position 4048 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000082965)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCTATGAACAGTGGAGTGAT
Hairpin Sequence:
5'-CCGG-CCCTATGAACAGTGGAGTGAT-CTCGAG-ATCACTCCACTGTTCATAGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCTATGAACAGTGGAGTGATCTCGAGATCACTCCACTGTTCATAGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCTATGAACAGTGGAGTGATCTCGAGATCACTCCACTGTTCATAGGG-3'

Other clones with same target sequence:

(none)