Construct: shRNA TRCN0000082968

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001835.1-4932s1c1
DNA Barcode:
CCCTACCTGCAGAGTTACTAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CLTCL1 (8218)
Gene Description:
clathrin heavy chain like 1
Transcript:
RefSeq NM_001835.1 (NON-CURRENT)
Match location:
Position 4932 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000082968)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCTACCTGCAGAGTTACTAA
Hairpin Sequence:
5'-CCGG-CCCTACCTGCAGAGTTACTAA-CTCGAG-TTAGTAACTCTGCAGGTAGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCTACCTGCAGAGTTACTAACTCGAGTTAGTAACTCTGCAGGTAGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCTACCTGCAGAGTTACTAACTCGAGTTAGTAACTCTGCAGGTAGGG-3'

Other clones with same target sequence:

(none)