Construct: shRNA TRCN0000082970

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001835.1-2408s1c1
DNA Barcode:
CCTATATTTATACCGCAACAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CLTCL1 (8218)
Gene Description:
clathrin heavy chain like 1
Transcript:
RefSeq NM_001835.1 (NON-CURRENT)
Match location:
Position 2408 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000082970)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTATATTTATACCGCAACAA
Hairpin Sequence:
5'-CCGG-CCTATATTTATACCGCAACAA-CTCGAG-TTGTTGCGGTATAAATATAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTATATTTATACCGCAACAACTCGAGTTGTTGCGGTATAAATATAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTATATTTATACCGCAACAACTCGAGTTGTTGCGGTATAAATATAGG-3'

Other clones with same target sequence:

(none)