Construct: shRNA TRCN0000083074

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_020655.2-2471s1c1
DNA Barcode:
CGCCATTCTGTTTATTAACTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
JPH3 (57338)
Gene Description:
junctophilin 3
Transcript:
RefSeq NM_020655.2 (NON-CURRENT)
Match location:
Position 2471 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000083074)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGCCATTCTGTTTATTAACTT
Hairpin Sequence:
5'-CCGG-CGCCATTCTGTTTATTAACTT-CTCGAG-AAGTTAATAAACAGAATGGCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGCCATTCTGTTTATTAACTTCTCGAGAAGTTAATAAACAGAATGGCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGCCATTCTGTTTATTAACTTCTCGAGAAGTTAATAAACAGAATGGCG-3'

Other clones with same target sequence:

(none)