Construct: shRNA TRCN0000083106

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_030882.2-951s1c1
DNA Barcode:
TGTTCTTACCTTAGTTGACAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
APOL2 (23780)
Gene Description:
apolipoprotein L2
Transcript:
RefSeq NM_030882.2 (NON-CURRENT)
Match location:
Position 951 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000083106)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGTTCTTACCTTAGTTGACAA
Hairpin Sequence:
5'-CCGG-TGTTCTTACCTTAGTTGACAA-CTCGAG-TTGTCAACTAAGGTAAGAACA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGTTCTTACCTTAGTTGACAACTCGAGTTGTCAACTAAGGTAAGAACATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGTTCTTACCTTAGTTGACAACTCGAGTTGTCAACTAAGGTAAGAACA-3'

Other clones with same target sequence:

  1. TRCN0000290384