Construct: shRNA TRCN0000083110

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_000329.1-1136s1c1
DNA Barcode:
CCCAACCTGAAGTTAGGAGAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RPE65 (6121)
Gene Description:
retinoid isomerohydrolase RPE65
Transcript:
RefSeq NM_000329.1 (NON-CURRENT)
Match location:
Position 1136 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000083110)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCAACCTGAAGTTAGGAGAT
Hairpin Sequence:
5'-CCGG-CCCAACCTGAAGTTAGGAGAT-CTCGAG-ATCTCCTAACTTCAGGTTGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCAACCTGAAGTTAGGAGATCTCGAGATCTCCTAACTTCAGGTTGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCAACCTGAAGTTAGGAGATCTCGAGATCTCCTAACTTCAGGTTGGG-3'

Other clones with same target sequence:

(none)