Construct: shRNA TRCN0000083134

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004543.2-17770s1c1
DNA Barcode:
GCCAGGTTAAATACCGAGAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NEB (4703)
Gene Description:
nebulin
Transcript:
RefSeq NM_004543.2 (NON-CURRENT)
Match location:
Position 17770 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000083134)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCAGGTTAAATACCGAGAAA
Hairpin Sequence:
5'-CCGG-GCCAGGTTAAATACCGAGAAA-CTCGAG-TTTCTCGGTATTTAACCTGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCAGGTTAAATACCGAGAAACTCGAGTTTCTCGGTATTTAACCTGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCAGGTTAAATACCGAGAAACTCGAGTTTCTCGGTATTTAACCTGGC-3'

Other clones with same target sequence:

(none)