Construct: shRNA TRCN0000083306

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_032122.3-670s1c1
DNA Barcode:
GAAACCTTCAAAGCTGAACTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
DTNBP1 (84062)
Gene Description:
dystrobrevin binding protein 1
Transcript:
RefSeq NM_032122.3 (NON-CURRENT)
Match location:
Position 670 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000083306)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAAACCTTCAAAGCTGAACTA
Hairpin Sequence:
5'-CCGG-GAAACCTTCAAAGCTGAACTA-CTCGAG-TAGTTCAGCTTTGAAGGTTTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAAACCTTCAAAGCTGAACTACTCGAGTAGTTCAGCTTTGAAGGTTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAAACCTTCAAAGCTGAACTACTCGAGTAGTTCAGCTTTGAAGGTTTC-3'

Other clones with same target sequence:

(none)