Construct: shRNA TRCN0000083332

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_000407.3-504s1c1
DNA Barcode:
CCTTGGGCTGCTGCACGCGTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
GP1BB (2812)
Gene Description:
glycoprotein Ib platelet subunit beta
Transcript:
RefSeq NM_000407.3 (NON-CURRENT)
Match location:
Position 504 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000083332)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTTGGGCTGCTGCACGCGTT
Hairpin Sequence:
5'-CCGG-CCTTGGGCTGCTGCACGCGTT-CTCGAG-AACGCGTGCAGCAGCCCAAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTTGGGCTGCTGCACGCGTTCTCGAGAACGCGTGCAGCAGCCCAAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTTGGGCTGCTGCACGCGTTCTCGAGAACGCGTGCAGCAGCCCAAGG-3'

Other clones with same target sequence:

(none)