Construct: shRNA TRCN0000083415

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_000516.3-474s1c1
DNA Barcode:
CCTCCCGAATTCTATGAGCAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
GNAS (2778)
Gene Description:
GNAS complex locus
Transcript:
RefSeq NM_000516.3 (NON-CURRENT)
Match location:
Position 474 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000083415)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTCCCGAATTCTATGAGCAT
Hairpin Sequence:
5'-CCGG-CCTCCCGAATTCTATGAGCAT-CTCGAG-ATGCTCATAGAATTCGGGAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTCCCGAATTCTATGAGCATCTCGAGATGCTCATAGAATTCGGGAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTCCCGAATTCTATGAGCATCTCGAGATGCTCATAGAATTCGGGAGG-3'

Other clones with same target sequence:

(none)