Construct: shRNA TRCN0000083446

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_000503.3-811s1c1
DNA Barcode:
CGACGGGTCTTTAAACAATTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
EYA1 (2138)
Gene Description:
EYA transcriptional coactivator and phosphatase 1
Transcript:
RefSeq NM_000503.3 (NON-CURRENT)
Match location:
Position 811 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000083446)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGACGGGTCTTTAAACAATTT
Hairpin Sequence:
5'-CCGG-CGACGGGTCTTTAAACAATTT-CTCGAG-AAATTGTTTAAAGACCCGTCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGACGGGTCTTTAAACAATTTCTCGAGAAATTGTTTAAAGACCCGTCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGACGGGTCTTTAAACAATTTCTCGAGAAATTGTTTAAAGACCCGTCG-3'

Other clones with same target sequence:

  1. TRCN0000315624