Construct: shRNA TRCN0000083633

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_013243.2-2611s1c1
DNA Barcode:
CCAGGCAGTAAGGTAGAAATA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SCG3 (29106)
Gene Description:
secretogranin III
Transcript:
RefSeq NM_013243.2 (NON-CURRENT)
Match location:
Position 2611 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000083633)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGGCAGTAAGGTAGAAATA
Hairpin Sequence:
5'-CCGG-CCAGGCAGTAAGGTAGAAATA-CTCGAG-TATTTCTACCTTACTGCCTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGGCAGTAAGGTAGAAATACTCGAGTATTTCTACCTTACTGCCTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGGCAGTAAGGTAGAAATACTCGAGTATTTCTACCTTACTGCCTGG-3'

Other clones with same target sequence:

(none)