Construct: shRNA TRCN0000083663

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_000231.1-1458s1c1
DNA Barcode:
CCCTTCAGTTTAATGCAAGTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SGCG (6445)
Gene Description:
sarcoglycan gamma
Transcript:
RefSeq NM_000231.1 (NON-CURRENT)
Match location:
Position 1458 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000083663)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCTTCAGTTTAATGCAAGTA
Hairpin Sequence:
5'-CCGG-CCCTTCAGTTTAATGCAAGTA-CTCGAG-TACTTGCATTAAACTGAAGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCTTCAGTTTAATGCAAGTACTCGAGTACTTGCATTAAACTGAAGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCTTCAGTTTAATGCAAGTACTCGAGTACTTGCATTAAACTGAAGGG-3'

Other clones with same target sequence:

(none)